DNA barcoding Clithon sp. (Gastropoda: Neritidae) from Badur Beach, Madura, Indonesia, based on COI gene molecular marker

Abstract. Djoemharsjah IS, Ambarwati R, Rahayu DA. 2023. DNA barcoding Clithonsp. (Gastropoda: Neritidae) from Badur Beach, Madura, Indonesia, based on COI gene molecular marker. Biodiversitas 24: xxxx. Neritidae is polymorphic with various shell colors and patterns; therefore, they were doubts about the identifying species genus Clithon, namely Clithon sp. Beach of Madura Island, Sumenep District, East Java Province, Indonesia. In addition, could provide genetic information using short sequences to quickly precisely identify species. This study aimed Cytochrome Oxidase subunit I(COI) genes analysis phylogenetic relationships. The research methods include sampling, sample preservation, morphological identification species, isolation, amplification, electrophoresis, sequencing Sanger method bioinformatics software. results barcode obtained a sequence length 490bp similarity value three samples between 96.75 98.97%. Barcode Life Data System consisted variations nucleotide bases, average distance in-group was 1.74% as Phylogenetic tree in same clade sowerbianum Récluz 1843 mertonianum Neighbor-Joining Tree Maximum Likelihood bootstrap values 96-100. Therefore, markers successfully identified Indonesia C. sowerbianum.